Toxicological studies

·    Cytotoxicity on cells of human origin
(fibroblasts, melanocytes, keratinocytes, adipocytes, endothelial cells …)

·    Phototoxicity on Balb/c 3T3 (OECD 432)

·    Skin irritation in reconstructed human epidermis (RhE)

·    Inflammatory potential in reconstructed human epidermis (RhE)

·    Apoptosis study

·    Ocular irritation in vitro (neutral red/SIRC or diffusion/agarose gel /L929 according to  OJ)

·    Acute toxicity in Mice (OECD 420)(*)

·    Skin irritation / Skin corrosion in Rabbits  (OECD 404)(*)

·    Ocular irritation / Ocular corrosion in Rabbits  (OECD 405)(*)

·    Sensitization in Guinea Pigs (OECD 406)(*)           

·    Pyrogenicity in Rabbits (European Pharmacopoeia)(*)

·    Sub-acute toxicity in Rats (4 weeks) (OECD 407)(*)

·    Sub-chronic toxicity in Rats (12 weeks) (OECD 408)(*)  

·    Carcinogenesis in Rats (OECD 451)(*)

·    Reproductive and developmental toxicity (OECD 415, OECD 416, OECD 414, OECD 421)(*)

Damage to DNA, mutagenicity, genotoxicity

·    Ames test 5 or 6 strains (Salmonella typhimurium, Escherichia coli) (OECD 471)

·    In vitro mammalian chromosome aberration test (human lymphocytes, CHO, V79, TK6)(OECD 473)

·    COMET assay (DNA degradation, DNA protection) (OECD 489)

·    In vitro mammalian cell gene mutation test (L5178Y, V79, TK6) (OCDE 476, OCDE 490)

·    In vitro mammalian micronucleus test (CHO, TK6) (OCDE 487)

·    Mammalian erythrocyte micronucleus test in Rats (OECD 474)(*)

In vitro assay of efficacy on cells of human origin and reconstructed human epidermis (RhE)

·    Cell growth

·    Cell differenciation

·    Synthesis (collagen, elastin) and deterioration (metalloproteases) of the extracellular matrix (ECM)

·    Anti-inflammatory activity (cytokine, prostaglandin)

·    Chemotaxis and cell migration

·    Pigmentant effect / depigmentant effect

·    Lipolytic effect

·    Scarring model

·    Cell renewal

·    Antiaging effect

·    Cytoskeleton study

·    Mitochondrial respiration

·    Protective effect (eg photoprotection)

·    Antiradical activity

·    Study of stress proteins

·    Stratum corneum functionality

·    Transcutaneous passage

·    Histology and immuno-histology (qualitative and quantitative)

·    Bactericidal effect

·    Search of contaminants (DNA, DNase,  RNase, ATP and RNA)

Screenings and adapted protocols on request

(*)Tests performed in partnership in compliance with Good Laboratory Practice

Zone de Texte: COSMETIC INGREDIENTS - COSMETIC INGREDIENTS - COSMETIC INGREDIENTS - COSMETIC INGREDIENTS - COSMETIC INGREDIENTS

Cosmetic ingredients

TOXICOLOGY - CELL AND TISSUE ENGINEERING - EXPERTISE - CONSULTING - - - - Over 25 years of experience...

© LEMI 2015 - LEGAL NOTICE

Lemi—laboratoire d’evaluation des materiels implantables